IGHV FISH Probe
The IGHV FISH probe is designed to hybridize to the IGHV gene and is primarily used for detecting amplifications and deletions associated with the gene. This probe is FISH confirmed on normal peripheral blood metaphase spreads and interphase nuclei. The probe can be labeled in one of five colors. Each probe is sold in a 20 test kit (approximately 20 slides - 22x22 mm area) and includes hybridization buffer. Please note that due to design optimizations, prices are subject to change.
** This product is for in vitro and research use only. This product is not intended for diagnostic use.
| SKU | Test Kits | Buffer | Dye Color | Order Now |
|---|---|---|---|---|
| IGH-20-OR (Standard Design) | 20 (40 μL) | 200 μL |  |
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| IGH-20-RE | 20 (40 μL) | 200 μL |  |
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| IGH-20-GO | 20 (40 μL) | 200 μL |  |
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| IGH-20-GR | 20 (40 μL) | 200 μL |  |
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| IGH-20-AQ | 20 (40 μL) | 200 μL |  |
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| IGH-20-DIG | 20 (40 μL) | 200 μL |  |
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| IGH-20-BIO | 20 (40 μL) | 200 μL |  |
Gene Summary
Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. This region represents the germline organization of the heavy chain locus. The locus includes V (variable), D (diversity), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single D segment with a J segment; this partially rearranged D-J gene is then joined to a V segment. The rearranged V-D-J is then transcribed with the IGHM constant region; this transcript encodes a mu heavy chain. Later in development B cells generate V-D-J-Cmu-Cdelta pre-messenger RNA, which is alternatively spliced to encode either a mu or a delta heavy chain. Mature B cells in the lymph nodes undergo switch recombination, so that the V-D-J gene is brought in proximity to one of the IGHG, IGHA, or IGHE genes and each cell expresses either the gamma, alpha, or epsilon heavy chain. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random addition of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Due to polymorphism, the numbers of functional V, J, and D genes differ among individuals and some V, D, J, and C segments may be pseudogenes. [provided by RefSeq, Dec 2017]
Gene Details
Gene Symbol: IGH
Gene Name: Immunoglobulin Heavy Locus
Chromosome: CHR14: 106879843-105586436
Locus: 14q32.33
FISH Probe Protocols
| Protocol, Procedure, or Form Name | Last Modified | Download |
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Customer Publications
Product Details
Product: IGHV FISH Probe
Test Kits: 20 (40 μL)
ISH Buffer: 200 μL
SKU: IGH-20-OR
Material Safety Data Sheet: MSDS.pdf
Turnaround Time: 7-10 Business Days
Shipping Time: 1-2 Day Expedited Shipping