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Chromogenic In Situ Hybridization

Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, whereas CISH probes are labelled with biotin or digoxigenin and can be detected using a bright-field microscope. CISH methodology may be used to evaluate gene amplification, gene deletion, chromosome translocation, and chromosome number. CISH utilizes conventional peroxidase or alkaline phosphatase reactions visualized under a standard bright-field microscope, and is applicable to formalin-fixed, paraffin-embedded (FFPE) tissues, blood or bone marrow smears, metaphase chromosome spreads, and fixed cells.

How Does CISH Compare To FISH & IHC?

  CISH FISH IHC
Microscope Bright-field Fluorescence Bright-field
Signal stability Archivable Fades over time Archivable
Magnification 40x 60–100x 20–40x
Morphology Good Limited Good
Amount of training required Medium High Low
Internal control Yes Yes No
Interpretation Objective/quantitative Objective/quantitative Subjective/qualitative

Amplification CISH Probes

Empire Genomics currently offers over 30,000 CISH probes that are used to detect the amplification of specific genes. Search for almost any human gene and chances are we can make a probe that covers it in as little as 10 days.

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