HBA-LCR FISH Probe
The HBA-LCR FISH probe is designed to hybridize to the HBA-LCR gene and is primarily used for detecting amplifications and deletions associated with the gene. This probe is FISH confirmed on normal peripheral blood metaphase spreads and interphase nuclei. The probe can be labeled in one of five colors. Each probe is sold in a 20 test kit (approximately 20 slides - 22x22 mm area) and includes hybridization buffer. Please note that due to design optimizations, prices are subject to change.
** This product is for in vitro and research use only. This product is not intended for diagnostic use.
| SKU | Test Kits | Buffer | Dye Color | Order Now |
|---|---|---|---|---|
| HBA-LCR-20-OR (Standard Design) | 20 (40 μL) | 200 μL |  |
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| HBA-LCR-20-RE | 20 (40 μL) | 200 μL |  |
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| HBA-LCR-20-GO | 20 (40 μL) | 200 μL |  |
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| HBA-LCR-20-GR | 20 (40 μL) | 200 μL |  |
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| HBA-LCR-20-AQ | 20 (40 μL) | 200 μL |  |
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| HBA-LCR-20-DIG | 20 (40 μL) | 200 μL |  |
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| HBA-LCR-20-BIO | 20 (40 μL) | 200 μL |  |
Gene Summary
This genomic region regulates developmental stage- and erythroid lineage-specific expression of the HBZ (hemoglobin, zeta), HBA2 (hemoglobin, alpha 2), HBA1 (hemoglobin, alpha 1) and HBQ1 (hemoglobin, theta 1) genes within the alpha-globin gene cluster. This region has properties of a locus control region (LCR) in that it can confer high-level and chromosomal position-independent expression on members of the alpha-globin gene cluster in a transgene assay, but unlike other LCRs, such as that regulating the beta-globin gene cluster, it lacks the ability to confer copy number-dependent expression on the linked genes. This region overlaps the NPRL3 (NPR3-like, GATOR1 complex subunit) gene, which is transcribed in the opposite orientation compared to the downstream alpha-globin genes. This regulatory region is characterized by multiple erythroid-specific DNase I hypersensitive sites, including HS-48, HS-40, HS-33, HS-10 and HS-8, where the HS-40 site represents an enhancer and is the major cis-acting regulatory element. HS-40 binds transcription factors and mediates looping with the promoters of the alpha-globin genes during erythroid development. The HS-40 element has also been used to enhance erythroid expression of beta-globin family members in gene therapy vectors for beta-chain hemoglobinopathies. Mutations in this regulatory region result in alpha thalassemias and alpha hemoglobinopathies. [provided by RefSeq, Aug 2015]
Gene Details
Gene Symbol: HBA-LCR
Gene Name: Alpha-globin Locus Control Region
Locus: 16p13.3
FISH Probe Protocols
| Protocol, Procedure, or Form Name | Last Modified | Download |
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Customer Publications
Product Details
Product: HBA-LCR FISH Probe
Test Kits: 20 (40 μL)
ISH Buffer: 200 μL
SKU: HBA-LCR-20-OR
Material Safety Data Sheet: MSDS.pdf
Turnaround Time: 7-10 Business Days
Shipping Time: 1-2 Day Expedited Shipping