A-GAMMA3'E FISH Probe
The A-GAMMA3'E FISH probe is designed to hybridize to the A-GAMMA3'E gene and is primarily used for detecting amplifications and deletions associated with the gene. This probe is FISH confirmed on normal peripheral blood metaphase spreads and interphase nuclei. The probe can be labeled in one of five colors. Each probe is sold in a 20 test kit (approximately 20 slides - 22x22 mm area) and includes hybridization buffer. Please note that due to design optimizations, prices are subject to change.
** This product is for in vitro and research use only. This product is not intended for diagnostic use.
| SKU | Test Kits | Buffer | Dye Color | Order Now |
|---|---|---|---|---|
| A-GAMMA3'E-20-OR (Standard Design) | 20 (40 μL) | 200 μL |  |
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| A-GAMMA3'E-20-RE | 20 (40 μL) | 200 μL |  |
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| A-GAMMA3'E-20-GO | 20 (40 μL) | 200 μL |  |
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| A-GAMMA3'E-20-GR | 20 (40 μL) | 200 μL |  |
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| A-GAMMA3'E-20-AQ | 20 (40 μL) | 200 μL |  |
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| A-GAMMA3'E-20-DIG | 20 (40 μL) | 200 μL |  |
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| A-GAMMA3'E-20-BIO | 20 (40 μL) | 200 μL |  |
Gene Summary
This genomic region represents regulatory elements that exist downstream of the hemoglobin subunit gamma 1 (HBG1) gene, which is also referred to as the A-gamma gene within the beta-globin gene cluster on chromosome 11. Two elements have been defined in this region, an enhancer termed Enh and a silencer termed F. The enhancer was shown to be active by transfection assays in both erythroleukemia and non-erythroid cell lines, and it may include both positively- and negatively-acting components. Both the Enh and F elements can negatively regulate transcription at the beta-globin locus in an embryonic-specific manner in some transgenic contexts, though they are not required for developmental switching from fetal gamma-globin to adult beta-globin gene expression in transgenic mice. These elements form an erythroid DNase I hypersensitive region with multiple sub-sites. This region can bind to multiple proteins, including the matrix-associated factor SATB1, the HOXB2 transcription factor and the erythroid GATA-1 binding protein. Mutations in this region may result in increased gamma-globin gene expression. These elements are deleted in some beta-thalassemia deletions that also remove the downstream adult beta-globin genes. [provided by RefSeq, Mar 2017]
Gene Details
Gene Symbol: A-GAMMA3'E
Gene Name: A-gamma-globin 3' Regulatory Region
Locus: 11p15.4
FISH Probe Protocols
| Protocol, Procedure, or Form Name | Last Modified | Download |
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Customer Publications
Product Details
Product: A-GAMMA3'E FISH Probe
Test Kits: 20 (40 μL)
ISH Buffer: 200 μL
SKU: A-GAMMA3'E-20-OR
Material Safety Data Sheet: MSDS.pdf
Turnaround Time: 7-10 Business Days
Shipping Time: 1-2 Day Expedited Shipping