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PCBP2-HMGA2 Fusion FISH Probe

The PCBP2-HMGA2 Fusion FISH Probe is used to confirm a fusion of the PCBP2 and HMGA2 genes. The fusion of the PCBP2 and HMGA2 genes has been associated with Skin Cutaneous Melanoma. These probes are FISH confirmed on normal peripheral blood in both interphase nuclei and metaphase spreads before shipment. Typical turnaround time for this product is 7-14 days after purchase.

** This product is for in vitro and research use only. This product is not intended for diagnostic use. Please note that both genes fall on the same chromosome and inter-chromosomal detection may be difficult to detect depending on the genes proximity to one another. Please consult our support staff before ordering this product to ensure that the probe can be designed to meet your specific needs.

Turnaround Time: 7-10 Business Days    Shipping Time: 1-2 Day Expedited Shipping
Download Datasheet Download FISH Protocol Download Material Safety Data Sheet

SKU Test Kits Buffer Dye Color Order Now
PCBP2-HMGA2-20-ORGR  (Standard Design) 20 (40 μL) 200 μL
PCBP2-HMGA2-20-RERE 20 (40 μL) 200 μL
PCBP2-HMGA2-20-REOR 20 (40 μL) 200 μL
PCBP2-HMGA2-20-REGO 20 (40 μL) 200 μL
PCBP2-HMGA2-20-REGR 20 (40 μL) 200 μL
PCBP2-HMGA2-20-REAQ 20 (40 μL) 200 μL
PCBP2-HMGA2-20-ORRE 20 (40 μL) 200 μL
PCBP2-HMGA2-20-OROR 20 (40 μL) 200 μL
PCBP2-HMGA2-20-ORGO 20 (40 μL) 200 μL
PCBP2-HMGA2-20-ORAQ 20 (40 μL) 200 μL
PCBP2-HMGA2-20-GORE 20 (40 μL) 200 μL
PCBP2-HMGA2-20-GOOR 20 (40 μL) 200 μL
PCBP2-HMGA2-20-GOGO 20 (40 μL) 200 μL
PCBP2-HMGA2-20-GOGR 20 (40 μL) 200 μL
PCBP2-HMGA2-20-GOAQ 20 (40 μL) 200 μL
PCBP2-HMGA2-20-GRRE 20 (40 μL) 200 μL
PCBP2-HMGA2-20-GROR 20 (40 μL) 200 μL
PCBP2-HMGA2-20-GRGO 20 (40 μL) 200 μL
PCBP2-HMGA2-20-GRGR 20 (40 μL) 200 μL
PCBP2-HMGA2-20-GRAQ 20 (40 μL) 200 μL
PCBP2-HMGA2-20-AQRE 20 (40 μL) 200 μL
PCBP2-HMGA2-20-AQOR 20 (40 μL) 200 μL
PCBP2-HMGA2-20-AQGO 20 (40 μL) 200 μL
PCBP2-HMGA2-20-AQGR 20 (40 μL) 200 μL
PCBP2-HMGA2-20-AQAQ 20 (40 μL) 200 μL

PCBP2 Gene Summary

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2018]

Gene Name: Poly(rC) Binding Protein 2

Chromosome: CHR12: 53845885 -53874946

Locus: 12q13.13

HMGA2 Gene Summary

This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Gene Name: High Mobility Group AT-hook 2

Chromosome: CHR12: 66218239 -66360071

Locus: 12q14.3

Gene Diseases

The PCBP2 HMGA2 Fusion has been associated with the following diseases:

Disease Name
Skin Cutaneous Melanoma

FISH Probe Protocols

Protocol, Procedure, or Form Name Last Modified Download

Epithelial-Myoepithelial Carcinoma Frequent Morphologic and Molecular Evidence of Preexisting Pleomorphic Adenoma, Common HRAS Mutations in PLAG1-intact and HMGA2-intact Cases, and Occasional TP53, FBXW7, and SMARCB1 Alterations in High-grade Cases

There is though to be a relationship between genetic aberrations, preexisting pleomorphic adenoma (PA), and the structural abnormality of epithelial-myoepithelial carcinomas (EMCAs). EMCAs were analyzed on a molecular level for PA by using FISH. FISH analysis was used with our break apart probes to detect PLAG1 and HMGA2 rearrangements. It was found that a relationship was present, as 80% of EMCA arise from PA, and EMCA genetics can vary with the status of PLAG1 and HMGA2.
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Recurrent rearrangements of the PLAG1 and HMGA2 genes in lacrimal gland pleomorphic adenoma and carcinoma ex pleomorphic adenoma

Lacrimal gland tumors are histologically similar to salivary gland tumors. In the salivary glands, pleomorphic adenoma (PA) and carcinoma ex pleomorphic adenoma (ca_ex_PA) are both characterized by PLAG1 and HMGA2 gene rearrangements. However, it is not known if these rearrangements are present in lacrimal gland PA and ca_ex_PA. FISH analysis was done with our PLAG1, HMGA2, and NFIB break apart probes. It was found that rearrangements were frequent in the tested lacrimal glands and that testing for the rearrangement can help in distinguishing lacrimal gland PA and ca_ex_PA from de novo carcinomas.
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Hydropic leiomyoma: a distinct variant of leiomyoma closely related to HMGA2 overexpression

Hydropic leiomyoma (HLM) is an understudied subtype of uterine leiomyoma (ULM). Little is known about HLM's etiology, genetics, and clinical profile. To account for this lack of data, the team analyzed 24 HLM cases for histology, immunohistochemistry and molecular alterations. Empire Genomics’ HMGA2 break apart probe was used to detect rearrangements of HMGA2, an abnormality previously reported in ULM. The gene was found rearranged in 32% of cases.
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Metastasizing Pleomorphic Adenoma: Recurrent PLAG1/HMGA2 Rearrangements and Identification of a Novel HMGA2-TMTC2 Fusion

Pleomorphic adenoma (PA), although considered benign, can undergo malignant transformation, and metastatic cases are well documented. There is a lack of research on genetic characterization of metastasizing versus benign PA. In this study, four cases of metastasizing PAs were cytogenetically profiled using both RNA sequencing and FISH. PLAG1 and HMGA2 break-apart probes from Empire Genomics were used to confirm rearrangements of the genes. PLAG1 rearrangements were detected in all four cases. Results demonstrated that MPA harbors the same disease-defining molecular hallmarks as their benign counterparts.
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Giant Cell Carcinosarcoma of the Parotid Gland With a PLAG1 Translocation in Association With a Pleomorphic Adenoma With HMGA2 Translocation: A Case Report and Fluorescence In Situ Hybridization Study

This case study looked at a parotid gland carcinosarcoma in a 77 year old patient. Our PLAG1 and HMGA2 break apart probes were used to detect rearrangements of the genes in both the carcinomatous and sarcomatous components of his tumor, to determine whether they harbored different genetic lesions. Both components were positive for PLAG1 and negative for HMGA2 translocations.
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