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NISH DAB Kit
NISH
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NISH DAB Kit

$750.00

SKU: DAB-KT- Categories: , , , , , Brand:

Kit Components

NISH DAB ChromogenX1

NISH DAB BufferX2

The NISH DAB Kit is a ready-to-use DAB chromogen system designed for use with HRP-based NISH detection assays in in situ hybridization (ISH) applications on formalin-fixed, paraffin-embedded (FFPE) tissue sections. The kit contains DAB (3,3'-diaminobenzidine) chromogen and DAB buffer, which together produce a stable, insoluble brown precipitate at the site of probe-target hybridization. The horseradish peroxidase (HRP) enzyme supplied by the NISH HRP Detection Kit catalyzes the oxidation of DAB, resulting in a permanent, localized signal that is easily visualized under brightfield microscopy. The NISH DAB Kit is supplied in pre-filled vials and is optimized for direct use with no mixing, dilution, or titration required. It is designed for use on the NeoPath Pro Automated Stainer and is compatible with the full portfolio of NISH Probes when paired with the NISH HRP Detection Kit. With 135 tests per kit, it supports high-throughput research workflows. For Research Use Only. Not for use in diagnostic procedures.

Product Specifications

Catalog Number
DAB-KT-135-R-NP
Pack Size
135 Tests
Detection Kit Needed
NISH HRP Kit (HRP-KT-40-R-NP)
Species Reactivity
Human
Sample Type
FFPE tissue sections
Application
In situ hybridization (ISH)
Protocol Name
Refer to NISH Probe datasheet
Storage
4°C, protected from light
Regulatory Status
For Research Use Only (RUO)
Technology
HCR™ Pro

Frequently Asked Questions (FAQ)

?What reagents are included in the NISH DAB Kit?

The kit includes DAB Chromogen (1 Ă— 1.5 mL) and DAB Buffer (2 Ă— 30 mL).

?Which detection system is used with the this chromogen kit?

The NISH DAB Kit is designed for use with the NISH HRP Detection Kit (HRP-KT-40-R-NP), which provides HRP-mediated signal amplification.

?Does the kit require any preparation before use?

No. All reagents are pre-filled and ready to use. No reconstitution, mixing, dilution, or titration is required.

?What instrument is required?

The kit is intended for use with the NeoPath Pro Automated Stainer (NPP0001).

Certificate of Analysis (CoA)

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Documentation

PDF icon NISH DAB Kit Datasheet

Customer Publications

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