The EsoFISH Multiplex FISH Probe from Empire Genomics enables researchers to visualize four genomic loci in a single fluorescence in situ hybridization (FISH) assay. Specifically, this quad-color multiplex probe targets MYC, P16 (CDKN2A), ERBB2, and ZNF217.

Each probe carries a distinct fluorophore—Aqua, Red, Green, and Gold. As a result, researchers can easily differentiate signals during fluorescence microscopy.

Because the probe combines four targets into one assay, it allows researchers to analyze multiple chromosomal regions simultaneously. In addition, this multiplex approach helps conserve valuable samples while streamlining experimental workflows.

Overall, the EsoFISH Probe supports efficient visualization of genomic loci while maintaining spatial resolution at the single-cell level.

Multiplex FISH Analysis

Multiplex fluorescence in situ hybridization allows researchers to examine several genomic targets within the same hybridization experiment. Consequently, scientists can evaluate multiple chromosomal loci without performing separate assays.

Furthermore, multiplex FISH preserves the spatial organization of DNA within individual cells. This feature allows researchers to study genomic regions while maintaining cellular context.

With four targets included in a single probe mix, the EsoFISH Probe simplifies experimental design and reduces the number of hybridizations required.

Gene Targets

The EsoFISH Multiplex FISH Probe includes four fluorescently labeled targets. Each probe binds to a specific genomic region and produces a distinct fluorescent signal.

MYC – Aqua
Targets the MYC gene region on chromosome 8.

P16 (CDKN2A) – Red
Targets the CDKN2A gene region, also known as P16, on chromosome 9.

ERBB2 – Green
Targets the ERBB2 gene region located on chromosome 17.

ZNF217 – Gold
Targets the ZNF217 gene region on chromosome 20.

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