Signal to Noise
Are Your Fluorophores Working for You—or Against You? In fluorescence in situ hybridization (FISH), performance is often judged by probe design, but in reality, fluorophore behavior is just as critical to signal quality. Even a well-designed probe can produce weak, unclear, or misleading results if the fluorophore is poorly matched to the instrument, filter set, […]
Fluorescence in situ hybridization (FISH) didn’t just change how we see the genome; it redefined the speed of clinical discovery. To understand where the technology is going, we have to look at the hurdles it has cleared over the last 50 years. The Milestones of Mapping The journey of FISH is a story of moving from the […]
If you’ve ever applied a FISH probe and watched it bead up instead of spreading evenly, you’re not alone—and it’s one of the most common issues labs encounter during hybridization. In this edition of Teagan’s Tech Tip, we break down why probe beading happens and, more importantly, how to prevent it to ensure consistent coverage […]
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