NISH Negative Control Probe
$200.00
Kit Components
Probe A
Probe B
The NISH Negative Control Probe is an in situ hybridization (ISH) reagent designed to assess non-specific background signal and confirm assay specificity in FFPE tissue sections. It targets the bacterial dapB gene — a widely adopted industry-standard negative control sequence with no complementary target present in human tissue.
When used as part of a properly controlled ISH workflow, the NISH Negative Control Probe allows researchers to distinguish true target-specific signal from non-specific probe binding or background staining. An absence of signal with this probe confirms that observed staining in experimental samples reflects genuine hybridization to the intended target rather than technical artifact.
Powered by HCR™ Pro, this probe delivers sensitive, high-clarity signal with minimal background. It is supplied ready-to-use in hybridization buffer and is compatible with the NISH HRP and AP Detection Kit and NISH DAB or Red Kit on the NeoPath Pro Automated Stainer. For Research Use Only. Not for use in diagnostic procedures.Product Specifications
Frequently Asked Questions (FAQ)
?What is the target of the NISH Negative Control Probe?
The probe targets dapB, a bacterial gene sequence with no homology to human nucleic acids, making it an industry-standard choice for confirming assay specificity in ISH workflows.
?What instrument is required?
The probe is intended for use with the NeoPath Pro Automated Stainer (NPP0001).
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