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Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation

2013-03-04 18:12:35

Nature Methods; 2013 Mar; 10(3):246-8


Marshall J Levesque and Arjun Raj



Abstract



We report intron chromosomal expression FISH (iceFISH), a multiplex imaging method for measuring gene expression and chromosome structure simultaneously on single chromosomes. We find substantial differences in transcriptional frequency between genes on a translocated chromosome and the same genes in their normal chromosomal context in the same cell. Correlations between genes on a single chromosome pointed toward a cis chromosome-level transcriptional interaction spanning 14.3 megabases.



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