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CELL PREPARATIONS AND CELL SUPPORTS AND THEIR USE IN THERANOSIS

2016-12-08 14:19:45

Free Patents Online; 08 December, 2016


Sokolova Irina, Schulz John, Kornfeld Olga, Panella Jeffrey, Song Minghao, Sitailo Svetlana, Policht Frank, Browne Gerry, Pestova Ekaterina



Abstract


A method of preparing cells from a patient specimen comprising a tissue or a clump of cells; a method of in situ hybridization (ISH) or immunohistochemistry (IHC); a method of theranosing a patient's response to an active agent that targets the tyrosine kinase pathway; an improvement of a method of theranosis comprising the method of ISH or IHC; a cell support on the surface of which are cells, which have been contacted with detectably labeled probes with the method of ISH or IHC; and a kit comprising (a) a cell support and/or at least one probe for ISH or IHC, and (b) instructions for carrying out the method of ISH or IHC on a cell support.



Introduction


A cell support comprising a tissue or clump of cells prepared from a patient specimen by a method comprising: (i) soaking a patient specimen in a pre-warmed, balanced salt solution comprising a reducing agent and a chelating agent; (ii) transferring the patient specimen to a fresh, balanced salt solution comprising a reducing agent, a source of calcium ions, and a polar aprotic solvent and mixing; (iii) removing the patient specimen from the fresh, balanced salt solution and either (a) centrifuging the solution to pellet disaggregated cells or (b) straining the solution to collect disaggregated cells; and (iv) after (iii)(a), discarding the supernatant, re-suspending the pelleted cells in residual supernatant, and placing the re-suspended cells in a fixative or, after (iii)(b), placing the strained cells in a fixative.



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